Dynamic changes of rat cartilage ultrastructure in the early process of papain-induced knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2015.18.001

Abstract

Abstract:

BACKGROUND: Papain-induced rat knee osteoarthritis is a common modeling method, which can obtain a stable osteoarthritis model.
OBJECTIVE: To observe the change of ultrastructure of chondrocytes in the early process of papain-induced rat knee osteoarthritis under transmission electron microscope.
METHODS: A total of 18 Sprague-Dawley rats were randomly divided into three groups. Two rats were considered as a normal control group, without intervention. The mixture of papain and L-cysteine was injected in right knee joint cavity of 16 rats to induce osteoarthritis models (osteoarthritis model group). Physiological saline was injected in the left side (physiological saline control group). At 1, 2, 4 and 6 weeks after injection, samples were collected. Transmission electron microscope was used to observe the change of cartilage ultrastructure of the medial femoral condyle joint.
RESULTS AND CONCLUSION: For the normal control group and physiological saline control group, their cytoplasm contained abundant rough endoplasmic reticulum and mitochondria. After 1 week of injection, mitochondria vacuoles and light expanded rough endoplasmic reticulum were visible. Two weeks later, lipid droplets appeared, mitochondria degeneration was distinct, vacuolization was serious and its number was reduced, and rough endoplasmic reticulum expansion was obvious. Four weeks later, lipid droplets became increased, and the
number of mitochondria decreased significantly. Most of the rough endoplasmic reticula were highly expanded, and part of the rough endoplasmic reticula were dissolved and fractured. Six weeks later, a number of lipid droplets were visible in cytoplasm, most of the mitochondria disappeared, only a small number of mitochondria existed, and most of the rough endoplasmic reticula were dissolved and fractured. These results confirmed that cartilage ultrastructure changes gradually in the early process of papain-induced rat knee osteoarthritis under transmission electron microscope.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Osteoarthritis, Cartilage, Papain, Microscopy, Electron, Transmission

CLC Number:

R318

Duan Wen-xiu1, Wang Zong-bao1, Zhang Hao1, Yang Zhi-wei1, Hu Zhi-lun1, Xu Fang-jun1, Xu Ya-lin2,. Dynamic changes of rat cartilage ultrastructure in the early process of papain-induced knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(18): 2789-2793.

Figures/Tables 1

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